Chitosan-Alginate Biocomposite Containing Fucoidan for Bone Tissue Engineering
نویسندگان
چکیده
Over the last few years, significant research has been conducted in the construction of artificial bone scaffolds. In the present study, different types of polymer scaffolds, such as chitosan-alginate (Chi-Alg) and chitosan-alginate with fucoidan (Chi-Alg-fucoidan), were developed by a freeze-drying method, and each was characterized as a bone graft substitute. The porosity, water uptake and retention ability of the prepared scaffolds showed similar efficacy. The pore size of the Chi-Alg and Chi-Alg-fucoidan scaffolds were measured from scanning electron microscopy and found to be 62-490 and 56-437 µm, respectively. In vitro studies using the MG-63 cell line revealed profound cytocompatibility, increased cell proliferation and enhanced alkaline phosphatase secretion in the Chi-Alg-fucoidan scaffold compared to the Chi-Alg scaffold. Further, protein adsorption and mineralization were about two times greater in the Chi-Alg-fucoidan scaffold than the Chi-Alg scaffold. Hence, we suggest that Chi-Alg-fucoidan will be a promising biomaterial for bone tissue regeneration.
منابع مشابه
Incorporation of Fucoidan in β-Tricalcium phosphate-Chitosan scaffold prompts the differentiation of human bone marrow stromal cells into osteogenic lineage
In our previous study, we reported the fabrication and characterization of a novel tricalcium phosphate-fucoidan-chitosan (TCP-Fu-Ch) biocomposite scaffold. However, the previous report did not show whether the biocomposite scaffold can exhibit osteogenic differentiation of human bone marrow stromal cells in osteogenic media and normal media supplemented with platelet-derived growth factor (PDG...
متن کاملEngineering cartilage or endochondral bone: a comparison of different naturally derived hydrogels.
Cartilaginous tissues engineered using mesenchymal stem cells (MSCs) have been shown to generate bone in vivo by executing an endochondral programme. This may hinder the use of MSCs for articular cartilage regeneration, but opens the possibility of using engineered cartilaginous tissues for large bone defect repair. Hydrogels may be an attractive tool in the scaling-up of such tissue engineered...
متن کاملHow calcite and modified hydroxyapatite influence physicochemical properties and cytocompatibility of alpha-TCP based bone cements
Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering. Recently a great deal of attention has been focused on calcium phosphate cements (CPCs) as the effective bone fillers. Despite a number of studies regarding CPCs, only a few compare the physicochemical and biological properties of α-TCP based materials of various pha...
متن کاملSynthesis and rheological evaluations of novel injectable sodium alginate/ chitosan-nanostructured hydroxyapatite composite bone pastes
In this paper, nanostructured carbonated hydroxyapatite powder was synthesized using biomimetic method. Physical properties and morphological features of the powder was studied using appropriate techniques such as BET, TEM, XRD and FTIR. The powder was separately mixed with 3 wt. % solutions of either sodium alginate or chitosan to form different pastes. Rheological behavior of the pastes in os...
متن کاملHydrogels Containing Marine Polysaccharides For Bone and Cartilage Tissue Engineering
Introduction: The most common biopolymers used to build-up 3D scaffolds for bone and cartilage tissue engineering are chitosan, alginate, cellulose derivatives, hyaluronic acid, chondroitin sulfate, collagen and gelatin. In this study, we propose to evaluate the potential of two new marine exopolysaccharides (HE800 and GY785) that are glycosaminoglycan-like polymers and that can be produced in ...
متن کامل